ABSTRACT
The present work aim to study the various pathogenic microorganisms from blood, wound, corneal and sputum samples from patients hosted in Tiruchirappalli area, Tamilnadu, India, during the month of February 2007 to June 2007. A total of 1031 samples were collected from patients. They include 195 blood samples, 310 sputum samples, 414 pus samples from wounds and 112 corneal swabs. The samples were inoculated into different culture media. Various bacterial and fungal colonies were isolated and identified by relevant staining methods and various biochemical utilization tests and also their antibiotics sensitivity were analyzed. Based on their antibiotic sensitivity pattern, bacterial and fungal pathogens were identified as sensitive and resistant strains. Mostly single pathogen was isolated from all the samples. Gram positive bacteria were found as common causative agent than gram negative bacteria. S. aureus and Klebsiella spp., were frequently isolated from all the samples. In blood samples, Klebsiella spp., E. coli, and Salmonella species were predominantly isolated, p-haemolytic Streptococci and S. aureus were predominantly isolated from sputum samples and Mycobacterium tuberculosis was isolated only in few samples. In wound samples S. aureus, Proteus mirabilis and P. vulgaris were predominantly isolated than E. coli, Enterobacter spp., P. aeruginosa, Serratia marcescens and Citrobacter divergens. Pseudomonas spp., Fusarium spp., Aspergillus Niger and A.flavus isolated from the corneal swabs. Antibiotic sensitivity tests were analyzed in those bacteria and fungal strains. The bacterial strains particularly S. aureus and Klebsiella spp., were highly resistant to 9 antibiotics where as E. coli, Enterobacter spp., and Streptococcus spp., were highly resistant to 10 antibiotics. The widespread uses of antibiotics are strongly associated with the development of bacterial drug resistance and also emerging of multidrug resistant strains